The current research of the Klocko lab involves understanding the genome organization of the filamentous fungus Neurospora crassa. Of particular interest is identifying the macromolecules critical for forming this non-stochastic organization, in which heterochromatic (silent) regions of the genome strongly interact to form a heterochromatin bundle and euchromatic (active) regions of the genome loop out from this bundle, and how this genome organization contributes to the regulation of gene expression.

• B.S., University of Maryland, 2002
• Ph.D., University of Wisconsin-Madison, 2009

Rodriguez S, Ward A, Reckard AT, Shtanko Y, Hull-Crew C, Klocko AD. The genome organization of Neurospora crassa at high resolution uncovers principles of fungal chromosome topology. G3 (Bethesda). 2022 May 6;12(5):jkac053.

Courtney AJ, Ferraro AR, Klocko AD*, Lewis ZA* “Chromatin Structure and Function in Neurospora crassa.” The Mycota, A Comprehensive Treatise on Fungi as Experimental Systems for Basic and Applied Research, Genetics and Biotechnology, third edition. Benz J.P., Schipper K. (eds). Vol 2. Springer. First online: October 29, 2020.

Klocko AD, Summers CA, Glover ML, Parrish R, Storck WK, McNaught KJ, Moss ND, Gotting K, Stewart A, Morrison AM, Payne L, Hatakeyama S, Selker EU. Selection and Characterization of Mutants Defective in DNA Methylation in Neurospora crassa. Genetics. 2020 Nov;216(3):671-688.

Klocko AD, Uesaka M, Ormsby T, Rountree MR, Wiles ET, Adhvaryu KK, Honda S, Selker EU. (2019) Nucleosome Positioning by an Evolutionarily Conserved Chromatin Remodeler Prevents Aberrant DNA Methylation in Neurospora. Genetics. 211(2):563-78. PMID: 30554169

Klocko AD, Ormsby T, Galazka JM, Leggett NA, Uesaka M, Honda S, et al. (2016) Normal chromosome conformation depends on subtelomeric facultative heterochromatin in Neurospora crassa. Proc Natl Acad Sci. 113(52):15048-53. PMID: 27856763

Galazka JM*, Klocko AD*, Uesaka M, Honda S, Selker EU, Freitag M. (2016) Neurospora chromosomes are organized by blocks of importin alpha-dependent heterochromatin that are largely independent of H3K9me3. Genome Res 26(8):1069-80. PMID: 27260477 *equal contribution

Klocko AD, Rountree MR, Grisafi PL, Hays SM, Adhvaryu KK, Selker EU. (2016) Neurospora importin α is required for normal heterochromatic formation and DNA methylation. PLoS Genet. 11(3):e1005083. PMID: 25793375

Klocko AD, Schroeder JW, Walsh BW, Lenhart JS, Evans ML, Simmons LA. (2011) Mismatch repair causes the dynamic release of an essential DNA polymerase from the replication fork. Mol Microbiol. 82(3):648-63. PMID: 21958350

Klocko AD, Wassarman KM. (2009) 6S RNA binding to Es⁷⁰ requires a positively charged surface of s⁷⁰ region 4.2. Mol Microbiol. 73(2):152-64. PMID: 19538447